KMID : 0616120160470020186
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Korean Journal of Pharmacognosy 2016 Volume.47 No. 2 p.186 ~ p.191
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A New Analysis of p-Coumaric Acid and Trans-cinnamic Acid in Propolis By UPLC
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Kim Se-Gun
Hong In-Pyo Woo Soon-Ok Jang Hye-Ri Han Sang-Mi
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Abstract
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A new UPLC-DAD method for simultaneous determination of p-coumaric acid and trans-cinnamic acid in propolis was developed using Halo-C18 column and step gradient elution of MeCN and 0.1%H3PO4 in 8 min. The method was validated by specificity, linearity, limit of detection, limit of quantification, precision(intra- and inter-day variability) and recovery tests. The validated method was sufficiently applicable for quantitative analysis of propolis products as well as propolis collected from different regions in Korea. The established method is simple, appropriate and efficient, and can be used for quality assessment of Korean propolis.
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KEYWORD
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Propolis, p-Coumaric acid, trans-Cinnamic acid, UPLC method
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